CIHR Group in Skeletal Development and Remodeling

2008 PUBLICATIONS

Stanton, L-A., Li, J., and Beier, F. (2008). PPARγ2 expression in growth plate chondrocytes is regulated by p38 and GSK-3. J Cell Mol Medicine, In press.
Teixeira, C., Agoston, H., and Beier, F. (2008). Nitric oxide, C-type natriuretic peptide and cGMP as regulators of endochondral ossification. Dev Biol, In press.
Gill, K.S., Beier, F., and Goldberg, H. (2008). Rho-Rock Signaling Differentially Regulates Chondrocyte Spreading on Fibronectin and Bone Sialoprotein. Am J Physiology: Cell Physiology Epub May 07, 2008.
McErlain DD, Appleton CT, Litchfield RB, Pitelka V, Henry JL, Bernier SM, Beier F, Holdsworth DW. (2008) Study of subchondral bone adaptations in a rodent surgical model of OA using in vivo micro-computed tomography. Osteoarthritis Cartilage 16(4):458-469.
Pala, D., Woods, A., Kennedy, L., Liu, S., Chen, S.,Bursell, L., Lyons, K.M., Carter, D.E., Beier, F., and Leask, A. (2008) Focal adhesion kinase/Src suppresses early chondrogenesis: central role of CCN2. J Biol Chem 283(14):9239-9247.
Solomon,L.A., Bérubé, N.G., and Beier, F. (2008) Transcriptionalregulators of chondrocyte hypertrophy. Birth Defects Research – EmbryoToday, In press.
Ulici, V., Hoenselaar, K., Gillespie, J.R., and Beier,F. (2008) PI3K/Akt signaling regulates chondrocyte proliferation,differentiation and apoptosis during endochondral bone growth. BMC Dev Biol 8:40.

2007 PUBLICATIONS

Bursell, L., Woods, A., James, C.G., Pala, D., Leask, A., and Beier, F. (2007) Src kinase inhibition promotes the chondrocyte phenotype. Arthritis Res Ther 9(5):R105.
Woods, A., Khan, S., and Beier, F. (2007) C-Type natriuretic peptide regulates cellular condensation and glycosaminoglycan synthesis during chondrogenesis. Endocrinology 148(10):5030-5041.
Appleton, C.T., Usmani, S.E., Bernier, S.M., Aigner, T. and Beier, F. (2007) Transforming growth factor alpha suppresses chondrocyte phenotype and Sox9 expression in experimental osteoarthritis. Arthritis Rheum 56(11):3693-3705.
James, C.G., Ulici, V., Tuckermann, J., Underhill, T.M. and Beier, F. (2007) Expression profiling of Dexamethasone-treated primary chondrocytes identifies targets of glucocorticoid signalling in endochondral bone development. BMC Genomics 8:205. [View HTML]
Pickles, M. and Leask, A. (2007) Analysis of the CCN2 promoter in PANC-1 cells: regulation by ras/MEK/ERK. J Cell Commun Signal In Press.
Shi-wen, X., Renzoni, E.A., Kennedy, L., Howat, S., Chen, Y., Pearson, J.D. Bou-Gharios, G., Dashwood, M.R., du Bois, R.M., Black, C.M., Denton, C.P., Abraham, D.J., Leask, A. (2007) Endogenous endothelin-1 signaling contributes to type I collagen and CCN2 overexpression in fibrotic fibroblasts. Matrix Biol In Press.
Woods, A., Wang, G., Dupuis, H., Shao, Z., and Beier, F. Rac1 Signaling Stimulates N-Cadherin expression, mesenchymal condensation and chondrogenesis. J Biol Chem In Press. [View HTML]
Chen, Y., Leask A., Abraham D.J., Pala D., Shi-wen, X., Khan, K., Liu, S., Carter, D.E., Wilcox-Adelman, S., Goetinck, P., Black, C.M., Pitsillides, A.M., Sarraf, C.E., Eastwood, M. (2007) Heparan sulfate-dependent ERK activation contributes to the over-expression of fibrotic proteins and the enhanced contraction by lesional dermal scleroderma fibroblasts of their extracellular matrix. Arthritis Rheum In press.
Liu, S., Shi-wen, X., Kennedy, L., Pala, D., Carter, DE, Black, CM, Abraham, DJ, Leask, A. (2007) FAK is required for TGFbeta-induced JNK phosphorylation in fibroblasts: implications for acquisition of a matrix remodeling phenotype. Mol Biol Cell 18(6):2169-2178. [View HTML] \| [PubMed]
Wang, G., Woods, A., Agoston, H., Ulici, V., Glogauer, M., and Beier, F. (2007) Genetic Ablation of Rac1 in Cartilage Results in Chondrodysplasia. Dev Biol 306:612-623. [View HTML]
Agoston, H., Khan, S., James, C.G., Gillespie, J.R., Serra, R., Stanton, L.-A., and Beier, F. (2007) C-type natriuretic peptide regulates endochondral bone growth through p38 MAP kinase-dependent and -independent pathways. BMC Developmental Biology 7:18. [View HTML]
Woods, A., Wang, G. and Beier, F. (2007) Regulation of Chondrocyte Differentiation by the Actin Cytoskeleton and Adhesive Interactions. J Cell Physiol In press. [PubMed]
Korcok, J., Sims, S. M. and Dixon, S. J. (2007) P2 nucleotide receptor signaling in osteoclasts. In: Nucleotides and Regulation of Bone Cell Function, edited by G. Burnstock and T. R. Arnett. CRC Press, Taylor & Francis Group. Boca Raton, Florida. pp. 25-59. (Book Chapter)
Zhang, H., Hunter, G.K., Goldberg, H.A., Lajoie. G.A., and Yeung. K.K-C. (2007) An Integrated Procedure of Selective Injection, Sample Stacking and Fractionation of Phosphopeptides for MALDI MS Analysis. Analytica Chimica Acta 581:268-280.
Appleton, C.T.G., Pitelka, V., Henry, J.L, and Beier, F. (2007) Global analyses of gene expression in early experimental osteoarthritis. *Arthritis Rheum* 56(6):1854-1868. [PubMed]
Appleton, C.T.G., McErlain, D.D, Pitelka, V., Schwartz, N., Bernier, S.M., Henry, J.L., Holdsworth, D.W., and Beier, F. (2007) Forced mobilization accelerates pathogenesis: Characterization of a pre-clinical surgical model of osteoarthritis. Arthritis Res Ther 9(1):R13. [PubMed]
Panupinthu, N., Zhao, L., Possmayer, F., Ke, H.Z., Sims, S.M., and Dixon, S.J. (2007) P2X7 Nucleotide Receptors Mediate Blebbing in Osteoblasts through a Pathway Involving Lysophosphatidic Acid. J Biol Chem 282(5):3403-3412. [PubMed]
Wazen, R.M., Tye C.E., Goldberg, H.A., Hunter, G.K., Smith, C.E., and Nanci, A. (2007) In vivo functional analysis of polyglutamic acid domains in recombinant bone sialoprotein. J Histochem Cytochem 55(1):35-42. [PubMed]
Armstrong, S., Korcok, J., Sims, S. M. and Dixon, S. J. (2007) Activation of transcription factors by extracellular nucleotides in immune and related cell types. Purinergic Signalling Published online: 3 January.
Kennedy, L., Liu, S., Shi-wen, X., Carter, D., Lyons, K., Black, C.M., Abraham, D.J, Leask A. (2007) CCN2 is essential for fibroblast function. Exp Cell Res 313(5):952-964. [PubMed]

2006 PUBLICATIONS
McCreary, C. R., Dixon, S. J., Fraher, L. J., Carson J. J. and Prato, F. S. (2006) Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle. Bioelectromagnetics 27:354-364.
Dragan, M., Dixon, S.J., Jaworski, E., Chan, T.S., O’Brien, P.J. and Wilson, J.X. (2006) Coenzyme Q1 depletes NAD(P)H and impairs recycling of ascorbate in astrocytes. Brain Res 1078:9-18.
Grohe, B., Rogers, K., Goldberg, H.A., and Hunter, G.K. (2006) Crystallization kinetics of calcium oxalate hydrates studied by scanning confocal interference microscopy. J Crystal Growth 295:148-157.
Agoston, H., Baybayan, L., and Beier, F. (2006) Dexamethasone stimulates expression of C-type natriuretic peptide in chondrocytes. BMC Musculoskelet Disord 7:87.
Ma, Q., Brown, M., Li, Y., Beier, F., and LuValle, P. (2007) Activating Transcription Factor 2 controls Bcl-2 promoter activity in growth plate chondrocytes. J Cell Biochem In press.
Roy, A.A., Nunn, C., Ming, H., Zou, M.X., Penninger, J., Kirshenbaum, L.A., Dixon, S.J., Chidiac, P. (2006) Upregulation of endogenous RGS2 mediates cross-desensitization between Gs and Gq signaling in osteoblasts. J Biol Chem 281(43):32684-32693.
Hoffman, L.M., Garcha, K., Karamboulas, K., Cowan, M.F., Drysdale, L.M., Horton, W.A., and Underhill, T.M. (2006) BMP action in skeletogenesis involves attenuation of retinoid signaling. J Cell Biol 174(1):101-113.
Bhattacharya M, Wang J, Ribeiro FM, Dixon SJ, Feldman RD, Hegele RA, Ferguson SS. Analysis of a missense variant of the human N-formyl peptide receptor that is associated with agonist-independent beta-arrestin association and indices of inflammation. Pharmacogenomics J In Press.
Stanton, L-A. and Beier, F. (2006) Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells. Exp Cell Res 313(1):146-155.
Koleganova, V.A., Bernier, S.M., Dixon, S.J., Rizkalla, A.S. (2006) Bioactive glass/polymer composite materials with mechanical properties matching those of cortical bone. J Biomed Mater Res A 77(3):572-579.
Kovac, J.R., Chrones, T., Preiksaitis, H.G., Sims, S.M. (2006) Tachykinin receptor expression and function in human esophageal smooth muscle. J Pharmacol Exp Ther 318(2):513-520.
Sathish, V., Xu, A., Karmazyn, M., Sims, S.M., and Narayanan, N. (2006) Mechanistic basis of differences in Ca2+ -handling properties of sarcoplasmic reticulum in right and left ventricles of normal rat myocardium. Am J Physiol Heart Circ Physiol 291(1):H88-H96.
Yaghi, A. and Sims, S.M. (2006) Constrictor-induced translocation of NFAT3 in human and rat pulmonary artery smooth muscle. Am J Physiol Lung Cell Mol Physiol 289(6):L1061-L1074.
James, C.G., Woods, A., Underhill, T.M., and Beier, F. (2006) The transcription factor ATF3 is upregulated during chondrocyte differentiation and represses cyclins D1 and A gene transcription. BMC Molecular Biology 7:30.
Leask, A. and Abraham, D.J. (2006) All in the CCN family: Essential matricellular signaling modulators emerge from the bunker J Cell Sci 119(Pt3):4803-4810.
Leask, A. (2006) TGF beta, cardiac fibroblasts, and the fibrotic response. Cardio Res In Press.
Leask, A. The identification of cell-type specific transcripts by DNA microarray analysis. (2006) Microvascular Research, Chapter 156, Elsevier 1296 pp. (Book Chapter)
Leask, A. (2006) Scar wars: Is TGF beta the phantom menace in scleroderma? Arth Res Ther 8(4):213.
Shi-wen, X., Rodriguez-Pascal, F., Lamas, S., Holmes, A., Howat, S., Pearson, J.D., Dashwood, M.R., du Bois, R.M., Denton, C.P., Black, C.M., Abraham, D.J., and Leask, A. (2006) Constitutive ALK5-independent JNK activation contributes to endothelin-1 over-expression in pulmonary fibrosis: Evidence of an autocrine endothelin loop operating through the endothelin A and B receptors. Mol Cell Biol 26(14):5518-5527.
Jones, D.H., Nakashima, T., Sanchez, O.H., Kozieradzki, I., Komarova, S.V., Sarosi, I., Morony, S., Rubin, E., Sarao, R., Komnenovic, V., Kong, Y.Y., Schreiber, M., Dixon, S.J., Sims, S.M., Khokha, R., Wada, T., and Penninger, J.M. (2006) Regulation of cancer cell migration and bone metastisis by RANKL. Nature 440:692-696.
Woods, A. and Beier, F. (2006) RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem 281(19):13134-13140.
Shi-wen, X., Stanton, L.A., Kennedy, L., Pala, D., Chen, Y., Howat, S.L., Renzoni, E.A., Carter, D.E., Bou-Gharios, G., Stratton, R., Pearson, J.D., Beier, F., Lyons, K.M., Black, C.M., Abraham, D.J., and Leask, A. (2006) CCN2 is necessary for adhesive responses to TGFb1 in embryonic fibroblasts. J Biol Chem 281:10715-10726.
Van Beek, J.P., Kennedy, L., Rockel, J.S., Bernier, S.M., Leask, A. (2006) The induction of CCN2 by TGFb1 requires Ets-1. Arth Res Ther 8:R36.
Appleton, C.T.G., James, C.G., and Beier, F. (2006) Regulator of G protein signaling (RGS) proteins differentially control chondrocyte differentiation. J Cell Physiol 207(3):725-745.


2005 PUBLICATIONS
Twiner, M. J., Chidiac, P., Dixon, S. J. and Trick, C. G. (2005) Extracellular organic compounds from the ichthyotoxic red tide alga Heterosigma akashiwo elevate cytosolic calcium and induce apoptosis in Sf9 cells. Harmful Algae 4:789-800.
Nicola, C., Timoshenko, A. V., Dixon, S. J., Lala, P. K. and Chakraborty, C. (2005) EP1 receptor-mediated migration of the first trimester human extravillous trophoblast: the role of intracellular calcium and calpain. J Clin Endocrinol Metab 90:4736-4746.
Chen, Y., Shiwen, X., Eastwood, M., Black, C.M., Denton, C.P., Leask, A., Abraham, D.J. ALK5 (TGF-B receptor type I) signaling contributes to the fibrotic phenotype of scleroderma fibroblasts. Arth Rheum 54:1309-1316.
Dziadzio, M., Usinger, W., Leask, A., Abraham, D., Black, C.M., Denton, D., Stratton, R. 2005. N-terminal CTGF is marker of fibrosis for the connective tissue disease scleroderma. QJM 98, 485-492.
Kovac, J.R., Preiksaitis, H.G., and Sims, S.M. (2005) Functional and molecular analysis of L-type calcium channels in human esophagus and lower esophageal sphincter smooth muscle. Am J Physiol Gastrointest Liver Physiol 289(6):G998-G1006.
Chen, Y., Shiwen, X., van Beek, J. Kennedy, L., McLeod, M., Renzoni, E.A., Bou-Gharios, G., Wilcox-Adelman, S., Goetinck, P.F., Eastwood, M., Black, C.M., Abraham, D.J., Leask, A. (2005) Matrix contraction by dermal fibroblasts requires TGFbeta/ALK5, heparan sulfate containing proteoglycans and MEK/ERK: Insights into pathological scarring in chronic fibrotic disease. Am J Pathol 167:1699-1711. *This is a recommended Faculty 1000 Paper.
James, CG, Appleton, CTG, Ulici, V, Underhill, TM, and Beier, F. (2005) Microarray analyses of gene expression during chondrocyte differentiation identifies novel regulators of hypertrophy. Mol Biol Cell 16(11):5316-5333.
Wang, G and Beier, F. (2005) Rac1/Cdc42 and RhoA GTPases antagonistically regulate chondrocyte proliferation, hypertrophy, and apoptosis. J Bone Miner Res 20(6), 1022-1031.
Korcok, J, Raimundo, LN, Du, X, Sims, SM, Dixon, SJ. (2005) P2Y6 nucleotide receptors activate NF-kappaB and increase survival of osteoclasts. J Biol Chem 280(17), 16909-16915.
Woods, A, Wang, G, and Beier, F. (2005) RhoA/ROCK signaling regulates Sox9 expression and actin organization during chondrogenesis. J Biol Chem 280(12), 11626-11634.
Tye, CE, Hunter, GK, and Goldberg, HA. (2005) Identification of the type I collagen-binding domain of bone sialoprotein and characterization of the mechanism of interaction. J Biol Chem 280(14), 13487-13492.
Komarova, SV, Pereverzev, A, Shum, JW, Sims, SM, and Dixon, SJ. (2005) Convergent signaling by acidosis and receptor activator of NF-kappaB ligand (RANKL) on the calcium/calcineurin/NFAT pathway in osteoclasts. Proc Natl Acad Sci USA 102(7), 2643-2648.
Beier, F. (2005) Cell-cycle control and the cartilage growth plate J Cell Physiol 202, 1-8.
Best, K. A., Holmes, M. E., Samson, S. E., Mwanjewe, J., Wilson, J. X., Dixon, S. J., and Grover, A. K. (2005) Ascorbate uptake in pig coronary artery endothelial cells Mol Cell Biochem 271, 43-9.
Denton, C. P., Lindahl, G. E., Khan, K., Xu, S. W., Ong, V. H., Gaspar, N. J., Lazaridis, K., Edwards, D. R., Leask, A., Eastwood, M., Leoni, P., Renzoni, E. A., Gharios, G. B., Abraham, D. J., and Black, C. M. (2005) Activation of key profibrotic mechanisms in transgenic fibroblasts expressing kinase-deficient type II transforming growth factor-beta receptor (T beta RII Delta k) Journal of Biological Chemistry 280, 16053-16065.
Keykhosravani, M., Doherty-Kirby, A., Zhang, C., Brewer, D., Goldberg, H. A., Hunter, G. K., and Lajoie, G. (2005) Comprehensive Identification of Post-translational Modifications of Rat Bone Osteopontin by Mass Spectrometry Biochemistry 44, 6990-7003.
Klooster, A. R., and Bernier, S. M. (2005) Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte Arthritis Res Ther 7, R127-38.
Komarova, S. V., Pereverzev, A., Shum, J. W., Sims, S. M., and Dixon, S. J. (2005) Convergent signaling by acidosis and receptor activator of NF-kappaB ligand (RANKL) on the calcium/calcineurin/NFAT pathway in osteoclasts Proc Natl Acad Sci U S A 102, 2643-8.
Leask, A., Holmes, A., Black, C. M., and Abraham, D. J. (2005) Connective tissue growth factor gene regulation. Requirements for its induction by transforming growth factor-ss 2 in fibroblasts (vol 278, pg, 13008, 2003) Journal of Biological Chemistry 280, 4004-4004.
Nohe, A., Keating, E., Underhill, T. M., Knaus, P., and Petersen, N. O. (2005) Dynamics and interaction of caveolin-1 isoforms with BMP-receptors J Cell Sci 118, 643-50.
Takayama, T., Suzuki, N., Narukawa, M., Goldberg, H. A., Otsuka, K., and Ito, K. (2005) Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone Life Sci 76, 1211-21.
Williams, B. A., Liu, C., Deyoung, L., Brock, G. B., and Sims, S. M. (2005) Regulation of intracellular Ca2+ release in corpus cavernosum smooth muscle: synergism between nitric oxide and cGMP Am J Physiol Cell Physiol 288, C650-8.

2004 PUBLICATIONS
Twiner, M.J., Dixon, S.J. and Trick, C.G. (2004) Extracellular organics from specific cultures of Heterosigma akashiwo (Raphidophyceae) irreversibly alter respiratory activity in mammalian cells. Harmful Algae 3:173-182.
Charras, G. T., Williams, B. A., Sims, S. M., and Horton, M. A. (2004) Estimating the sensitivity of mechanosensitive ion channels to membrane strain and tension Biophys J 87, 2870-84.
Chen, Y. L., Abraham, D. J., Xu, S. W., Pearson, J. D., Black, C. M., Lyons, K. M., and Leask, A. (2004) CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin Molecular Biology of the Cell 15, 5635-5646.
Dixon, S. J., Yu, R., Panupinthu, N., and Wilson, J. X. (2004) Activation of P2 nucleotide receptors stimulates acid efflux from astrocytes Glia 47, 367-76.
Halawani, D., Mondeh, R., Stanton, L. A., and Beier, F. (2004) p38 MAP kinase signaling is necessary for rat chondrosarcoma cell proliferation Oncogene 23, 3726-3731.
Holmes, A. M., Howat, S., Xu, S. W., Leask, A., Ponticos, M., Pearson, J., Abraham, D. J., and Black, C. M. (2004) Transcriptional profiling of the scleroderma fibroblast: Evidence of a dysregulated response to TGF ss Arthritis and Rheumatism 50, S624-S624.
Jay, K. E., Rouleau, A., Underhill, T. M., and Bhatia, M. (2004) Identification of a novel population of human cord blood cells with hema-topoietic and chondrocytic potential Cell Res 14, 268-82.
Korcok, J., Raimundo, L. N., Ke, H. Z., Sims, S. M., and Dixon, S. J. (2004) Extracellular nucleotides act through P2X7 receptors to activate NF-kappaB in osteoclasts J Bone Miner Res 19, 642-51.
Leask, A., and Abraham, D. J. (2004) TGF-beta signaling and the fibrotic response Faseb Journal 18, 816-827.
Leask, A., Denton, C. P., and Abraham, D. J. (2004) Insights into the molecular mechanism of chronic fibrosis: The role of connective tissue growth factor in scleroderma Journal of Investigative Dermatology 122, 1-6.
Nanci, A., Wazen, R. M., Zalzal, S. F., Fortin, M., Goldberg, H. A., Hunter, G. K., and Ghitescu, D. L. (2004) A tracer study with systemically and locally administered dinitrophenylated osteopontin J Histochem Cytochem 52, 1591-600.
Natale, D. R., Paliga, A. J. M., Beier, F., D'Souza, S. J. A., and Watson, A. J. (2004) p38 MAPK signaling during murine preimplantation development Developmental Biology 268, 76-88.
Ozog, M. A., Bernier, S. M., Bates, D. C., Chatterjee, B., Lo, C. W., and Naus, C. C. (2004) The complex of ciliary neurotrophic factor-ciliary neurotrophic factor receptor alpha up-regulates connexin43 and intercellular coupling in astrocytes via the Janus tyrosine kinase/signal transducer and activator of transcription pathway Mol Biol Cell 15, 4761-74.
Pampena, D. A., Robertson, K. A., Litvinova, O., Lajoie, G., Goldberg, H. A., and Hunter, G. K. (2004) Inhibition of hydroxyapatite formation by osteopontin phosphopeptides Biochem J 378, 1083-7.
Poustie, M. W., Carran, J., McEleney, K., Dixon, S. J., Anastassiades, T. P., and Bernier, S. M. (2004) N-butyryl glucosamine increases matrix gene expression by chondrocytes J Pharmacol Exp Ther 311, 610-6.
Renzoni, E. A., Abraham, D. J., Howat, S., Xu, S. W., Sestini, P., Bou-Gharios, G., Wells, A. U., Veeraraghavan, S., Nicholson, A. G., Denton, C. P., Leask, A., Pearson, J. D., Black, C. M., Welsh, K. I., and du Bois, R. M. (2004) Gene expression profiling reveals novel TGF beta targets in adult lung fibroblasts Respiratory Research 5, -.
Stanton, L. A., Sabari, S., Sampaio, A. V., Underhill, T. M., and Beier, F. (2004) p38 MAP kinase signalling is required for hypertrophic chondrocyte differentiation Biochem J 378, 53-62.
Wang, G. Y., Woods, A., Sabari, S., Pagnotta, L., Stanton, L. A., and Beier, F. (2004) RhoA/ROCK signaling suppresses hypertrophic chondrocyte differentiation Journal of Biological Chemistry 279, 13205-13214.
Woods, A., James, C., Underhill, T. M., and Beier, F. (2004) Identification of the putative collagen X gene from the pufferfish Fugu rubripes Gene 342, 77-83.
Xu, S. W., Howat, S. L., Renzoni, E. A., Holmes, A., Pearson, J. D., Dashwood, M. R., Bou-Gharios, G., Denton, C. P., du Bois, R. M., Black, C. M., Leask, A., and Abraham, D. J. (2004) Endothelin-1 induces expression of matrix-associated genes in lung fibroblasts through MEK/ERK Journal of Biological Chemistry 279, 23098-23103.
Zhu, B., Suzuki, K., Goldberg, H. A., Rittling, S. R., Denhardt, D. T., McCulloch, C. A., and Sodek, J. (2004) Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-protein-coupled receptors: evidence of a role for an intracellular form of osteopontin J Cell Physiol 198, 155-67.

2003 PUBLICATIONS
Brown, K., Chen, Y., Underhill, T. M., Mymryk, J. S., and Torchia, J. (2003) The coactivator p/CIP/SRC-3 facilitates retinoic acid receptor signaling via recruitment of GCN5 J Biol Chem 278, 39402-12.
Chakraborty, C., Barbin, Y. P., Chakrabarti, S., Chidiac, P., Dixon, S. J., and Lala, P. K. (2003) Endothelin-1 promotes migration and induces elevation of [Ca2+]i and phosphorylation of MAP kinase of a human extravillous trophoblast cell line Mol Cell Endocrinol 201, 63-73.
Hoffman, L. M., Weston, A. D., and Underhill, T. M. (2003) Molecular mechanisms regulating chondroblast differentiation J Bone Joint Surg Am85-A Suppl 2, 124-32.
Holmes, A., Abraham, D. J., Chen, Y. J., Denton, C., Xu, S. W., Black, C. M., and Leask, A. (2003) Constitutive connective tissue growth factor expression in scleroderma fibroblasts is dependent on Sp1 Journal of Biological Chemistry 278, 41728-41733.
Karkanis, T., DeYoung, L., Brock, G. B., and Sims, S. M. (2003) Ca2+-activated Cl- channels in corpus cavernosum smooth muscle: a novel mechanism for control of penile erection J Appl Physiol 94, 301-13.
Karkanis, T., Li, S., Pickering, J. G., and Sims, S. M. (2003) Plasticity of KIR channels in human smooth muscle cells from internal thoracic artery Am J Physiol Heart Circ Physiol 284, H2325-34.
Ke, H. Z., Qi, H., Weidema, A. F., Zhang, Q., Panupinthu, N., Crawford, D. T., Grasser, W. A., Paralkar, V. M., Li, M., Audoly, L. P., Gabel, C. A., Jee, W. S., Dixon, S. J., Sims, S. M., and Thompson, D. D. (2003) Deletion of the P2X7 nucleotide receptor reveals its regulatory roles in bone formation and resorption Mol Endocrinol 17, 1356-67.
Komarova, S. V., Pilkington, M. F., Weidema, A. F., Dixon, S. J., and Sims, S. M. (2003) RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor kappa B in osteoclasts J Biol Chem 278, 8286-93.
Komarova, S. V., Shum, J. B., Paige, L. A., Sims, S. M., and Dixon, S. J. (2003) Regulation of osteoclasts by calcitonin and amphiphilic calcitonin conjugates: role of cytosolic calcium Calcif Tissue Int 73, 265-73.
Komarova, S. V., Smith, R. J., Dixon, S. J., Sims, S. M., and Wahl, L. M. (2003) Mathematical model predicts a critical role for osteoclast autocrine regulation in the control of bone remodeling Bone 33, 206-15.
Korcok, J., Dixon, S. J., Lo, T. C., and Wilson, J. X. (2003) Differential effects of glucose on dehydroascorbic acid transport and intracellular ascorbate accumulation in astrocytes and skeletal myocytes Brain Res 993, 201-7.
Leask, A., and Abraham, D. J. (2003) The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology Biochemistry and Cell Biology-Biochimie Et Biologie Cellulaire 81, 355-363.
Leask, A., Holmes, A., Black, C. M., and Abraham, D. J. (2003) Connective tissue growth factor gene regulation - Requirements for its induction by transforming growth factor-beta 2 in fibroblasts Journal of Biological Chemistry 278, 13008-13015.
Leask, A., Holmes, A. M., Pontocos, M., Rajkumar, V., Shiwen, X., Stratton, R., Abraham, D., and Black, C. (2003) Mechanism underlying the overexpression of CTGF in scleroderma fibroblasts: Role in elevated expression in scleroderma phenotype. Arthritis and Rheumatism 48, S155-S155.
Liu, J., Chakraborty, C., Graham, C. H., Barbin, Y. P., Dixon, S. J., and Lala, P. K. (2003) Noncatalytic domain of uPA stimulates human extravillous trophoblast migration by using phospholipase C, phosphatidylinositol 3-kinase and mitogen-activated protein kinase Exp Cell Res 286, 138-51.
Luvalle, P., Ma, Q., and Beier, F. (2003) The role of activating transcription factor-2 in skeletal growth control J Bone Joint Surg Am85-A Suppl 2, 133-6.
Nohe, A., Keating, E., Underhill, T. M., Knaus, P., and Petersen, N. O. (2003) Effect of the distribution and clustering of the type I A BMP receptor (ALK3) with the type II BMP receptor on the activation of signalling pathways J Cell Sci 116, 3277-84.
Ponticos, M., Holmes, A. M., Rajkumar, V., Wen, X. S., Leask, A., Abraham, D. J., and Black, C. M. (2003) Connective tissue growth factor (CTGF) mediates extracellular matrix deposition in bleomycin-induced lung fibrosis via the MEK/ERK signaling pathways. Arthritis and Rheumatism 48, S156-S156.
Seguin, C. A., and Bernier, S. M. (2003) TNFalpha suppresses link protein and type II collagen expression in chondrocytes: Role of MEK1/2 and NF-kappaB signaling pathways J Cell Physiol 197, 356-69.
Shiwen, X., Dashwood, M. R., Denton, C. P., Renzoni, E. A., Bou-Gharios, G., du Bois, R., Leask, A., Abraham, D., and Black, C. M. (2003) Response of human lung fibroblast cells to stimulation by endothelin-1: Involvement of ras/MEK/ERK and PI3 kinase Arthritis and Rheumatism 48, S39-S39.
Stanton, L. A., Underhill, T. M., and Beier, F. (2003) MAP kinases in chondrocyte differentiation Dev Biol 263, 165-75.
Tye, C. E., Rattray, K. R., Warner, K. J., Gordon, J. A., Sodek, J., Hunter, G. K., and Goldberg, H. A. (2003) Delineation of the hydroxyapatite-nucleating domains of bone sialoprotein J Biol Chem 278, 7949-55.
Wang, J., Laurier, L. G., Sims, S. M., and Preiksaitis, H. G. (2003) Enhanced capacitative calcium entry and TRPC channel gene expression in human LES smooth muscle Am J Physiol Gastrointest Liver Physiol 284, G1074-83.
Weston, A. D., Blumberg, B., and Underhill, T. M. (2003) Active repression by unliganded retinoid receptors in development: less is sometimes more J Cell Biol 161, 223-8.
Weston, A. D., Hoffman, L. M., and Underhill, T. M. (2003) Revisiting the role of retinoid signaling in skeletal development Birth Defects Res C Embryo Today 69, 156-73.
Weston, A. D., Sampaio, A. V., Ridgeway, A. G., and Underhill, T. M. (2003) Inhibition of p38 MAPK signaling promotes late stages of myogenesis J Cell Sci116, 2885-93.
Wongtrakool, C., Malpel, S., Gorenstein, J., Sedita, J., Ramirez, M. I., Underhill, T. M., and Cardoso, W. V. (2003) Down-regulation of retinoic acid receptor alpha signaling is required for sacculation and type I cell formation in the developing lung J Biol Chem 278, 46911-8.
Xu, S. W., Dashwood, M. R., Denton, C. P., Renzoni, E. A., Bou-Gharis, G., du Bois, R., Leask, A., Abraham, D., and Black, C. M. (2003) Response of human lung fibroblast cells to stimulation by endothelin-1: Involvement of ras/MEK/ERK and p13 kinase. Arthritis and Rheumatism 48, S156-S156.
Yang, J., Bernier, S. M., Ichim, T. E., Li, M., Xia, X., Zhou, D., Huang, X., Strejan, G. H., White, D. J., Zhong, R., and Min, W. P. (2003) LF15-0195 generates tolerogenic dendritic cells by suppression of NF-kappaB signaling through inhibition of IKK activity J Leukoc Biol 74, 438-47.

2002 PUBLICATIONS
Beier, F., and LuValle, P. (2002) The cyclin D1 and cyclin A genes are targets of activated PTH/PTHrP receptors in Jansen's metaphyseal chondrodysplasia Mol Endocrinol 16, 2163-73.
Blom, I. E., Goldschmeding, R., and Leask, A. (2002) Gene regulation of connective tissue growth factor: new targets for antifibrotic therapy Matrix Biology 21, 473-482.
Chen, Y. J., Blom, I. E., Sa, S., Goldschmeding, R., Abraham, D. J., and Leask, A. (2002) CTGF expression in mesangial cells: Involvement of SMADs, MAP kinase, and PKC Kidney International 62, 1149-1159.
Deshpande, M. A., Wang, J., Preiksaitis, H. G., Laurier, L. G., and Sims, S. M. (2002) Characterization of a voltage-dependent Na(+) current in human esophageal smooth muscle Am J Physiol Cell Physiol 283, C1045-55.
Holmes, A., Leask, A., Ponticos, M., Finlay, D. R., Pennington, D., Wen, X. S., Findell, P. R., Connolly, K., Chen, Y., Venstrom, K., Dou, X., Jackman, J. K., Abraham, D. J., and Black, C. M. (2002) Dysregulated TGF-beta signalling in scleroderma fibroblasts and the effect of over-expression of endoglin. Arthritis and Rheumatism 46, S388-S388.
Holmes, M. E., Mwanjewe, J., Samson, S. E., Haist, J. V., Wilson, J. X., Dixon, S. J., Karmazyn, M., and Grover, A. K. (2002) Dehydroascorbic acid uptake by coronary artery smooth muscle: effect of intracellular acidification Biochem J 362, 507-12.
Leask, A., Abraham, D. J., Finlay, D. R., Holmes, A., Pennington, D., ShiWen, X., Chen, Y., Venstrom, K., Dou, X., Ponticos, M., Black, C., Bernabeu, C., Jackman, J. K., Findell, P. R., and Connolly, M. K. (2002) Dysregulation of transforming growth factor beta signaling in scleroderma - Overexpression of endoglin in cutaneous scleroderma fibroblasts (vol 46, pg 1857, 2002) Arthritis and Rheumatism 46, 2830-2830.
Leask, A., Abraham, D. J., Finlay, D. R., Holmes, A., Pennington, D., Shi-Wen, X., Chen, Y., Venstrom, K., Dou, X., Ponticos, M., Black, C., Jackman, J. K., Findell, P. R., and Connolly, M. K. (2002) Dysregulation of transforming growth factor beta signaling in scleroderma - Overexpression of endoglin in cutaneous scleroderma fibroblasts Arthritis and Rheumatism 46, 1857-1865.
Leask, A., Stratton, R., Rajkumar, V., Ponticos, M., Nichols, B., Shiwen, X., Black, C., and Abraham, D. (2002) Prostacyclin derivatives prevent the fibrotic response to TGF beta 2 by inhibiting the Ras/MEK/ERK pathway Molecular Biology of the Cell 13, 430a-430a.
Lui, K. E., Panchal, A. S., Santhanagopal, A., Dixon, S. J., and Bernier, S. M. (2002) Epidermal growth factor stimulates proton efflux from chondrocytic cells J Cell Physiol 192, 102-12.
Mason, J. M., Xu, H. P., Rao, S. K., Leask, A., Barcia, M., Shan, J. D., Stephenson, R., and Tabibzadeh, S. (2002) Lefty contributes to the remodeling of extracellular matrix by inhibition of connective tissue growth factor and collagen mRNA expression and increased proteolytic activity in a fibrosarcoma model Journal of Biological Chemistry 277, 407-415.
Ponticos, M., Holmes, A. M., Wen, X. S., Rajkumar, V., Leask, A., Abraham, D. J., and Black, C. M. (2002) Connective Tissue Growth Factor (CTGF) expression in response to injury mediates matrix deposition in bleomycin-induced lung fibrosis. Arthritis and Rheumatism 46, S354-S355.
Riemer, S., Gebhard, S., Beier, F., Poschl, E., and von der Mark, K. (2002) Role of c-fos in the regulation of type X collagen gene expression by PTH and PTHrP: localization of a PTH/PTHrP-responsive region in the human COL10A1 enhancer J Cell Biochem 86, 688-99.
Stanton, L., Sabari, S., and Beier, F. (2002) p38 mitogen-activated protein kinases in chondrocyte differentiation. Journal of Bone and Mineral Research 17, S402-S402.
Suzuki, K., Zhu, B., Rittling, S. R., Denhardt, D. T., Goldberg, H. A., McCulloch, C. A., and Sodek, J. (2002) Colocalization of intracellular osteopontin with CD44 is associated with migration, cell fusion, and resorption in osteoclasts J Bone Miner Res 17, 1486-97.
Weston, A. D., Chandraratna, R. A., Torchia, J., and Underhill, T. M. (2002) Requirement for RAR-mediated gene repression in skeletal progenitor differentiation J Cell Biol 158, 39-51.

2001 PUBLICATIONS
Naemsch, L.N., Du, X., Sims, S.M., and Dixon S.J. (2001) P2 nucleotide receptors in osteoclasts. Drug Dev Res 53:130-139.
Twiner, M.J., Dixon, S.J. and Trick, C.G. (2001) Toxic effects of Heterosigma akashiwo do not appear to be mediated by hydrogen peroxide. Limnol Oceanogr 46:1400-1405.
Beier, F., Ali, Z., Mok, D., Taylor, A. C., Leask, T., Albanese, C., Pestell, R. G., and LuValle, P. (2001) TGFbeta and PTHrP control chondrocyte proliferation by activating cyclin D1 expression Mol Biol Cell 12, 3852-63.
Chen, Y. J., Segarini, P., Raoufi, F., Bradham, D., and Leask, A. (2001) Connective tissue growth factor is secreted through the Golgi and is degraded in the endosome Experimental Cell Research 271, 109-117.
Goldberg, H. A., Warner, K. J., Li, M. C., and Hunter, G. K. (2001) Binding of bone sialoprotein, osteopontin and synthetic polypeptides to hydroxyapatite Connect Tissue Res 42, 25-37.
Hardy, D. B., Dixon, S. J., Narayanan, N., and Yang, K. (2001) Calcium inhibits human placental 11beta-hydroxysteroid dehydrogenase type 2 activity Biochem Biophys Res Commun 283, 756-61.
Holmes, A., Abraham, D. J., Sa, S., Xu, S. W., Black, C. M., and Leask, A. (2001) CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling Journal of Biological Chemistry 276, 10594-10601.
Hunter, G. K., Poitras, M. S., Underhill, T. M., Grynpas, M. D., and Goldberg, H. A. (2001) Induction of collagen mineralization by a bone sialoprotein--decorin chimeric protein J Biomed Mater Res 55, 496-502.
Jacenko, O., Tao, Z., Campbell, M., Roberts, D., Beier, F., and LuValle, P. (2001) Linking endochondral ossification to hematopoiesis Faseb Journal 15, A1100-A1100.
Karkanis, T., Jiao, Y., Hurley, B. R., Li, S., Pickering, J. G., and Sims, S. M. (2001) Functional receptor-channel coupling compared in contractile and proliferative human vascular smooth muscle J Cell Physiol 187, 244-55.
Komarova, S. V., Dixon, S. J., and Sims, S. M. (2001) Osteoclast ion channels: potential targets for antiresorptive drugs Curr Pharm Des 7, 637-54.
Leask, A., Sa, S., Chen, Y. J., Holmes, A., Xu, S. W., Stratton, R., Black, C., and Abraham, D. (2001) Connective tissue growth factor (CTGF) and fibrosis Molecular Biology of the Cell 12, 461a-461a.
Leask, A., Sa, S., Holmes, A., Shiwen, X., Black, C. M., and Abraham, D. J. (2001) The control of ccn2(ctgf) gene expression in normal and scleroderma fibroblasts Journal of Clinical Pathology-Molecular Pathology 54*, 180-183 .
Li, S., Fan, Y. S., Chow, L. H., Van Den Diepstraten, C., van Der Veer, E., Sims, S. M., and Pickering, J. G. (2001) Innate diversity of adult human arterial smooth muscle cells: cloning of distinct subtypes from the internal thoracic artery Circ Res 89, 517-25.
Naemsch, L. N., Dixon, S. J., and Sims, S. M. (2001) Activity-dependent development of P2X7 current and Ca2+ entry in rabbit osteoclasts J Biol Chem 276, 39107-14.
Pilkington, M. F., Sims, S. M., and Dixon, S. J. (2001) Transforming growth factor-beta induces osteoclast ruffling and chemotaxis: potential role in osteoclast recruitment J Bone Miner Res 16, 1237-47.
Santhanagopal, A., Chidiac, P., Horne, W. C., Baron, R., and Dixon, S. J. (2001) Calcitonin (CT) rapidly increases NA(+)/H(+) exchange and metabolic acid production: effects mediated selectively by the C1A CT receptor isoform Endocrinology 142, 4401-13.
Stratton, R., Xu, S. W., Martini, G., Holmes, A., Leask, A., Haberberger, T., Martin, G. R., Black, C. M., and Abraham, D. (2001) Iloprost suppresses connective tissue growth factor production in fibroblasts and in the skin of scleroderma patients Journal of Clinical Investigation 108, 241-250.
Underhill, D. A., Vogan, K. J., Underhill, T. M., and Gros, P. (2001) Identification of a novel, alternatively spliced isoform and single nucleotide polymorphisms in the murine Pea-15 gene Mamm Genome 12, 172-4.
Underhill, T. M., Sampaio, A. V., and Weston, A. D. (2001) Retinoid signalling and skeletal development Novartis Found Symp 232, 171-85; discussion 185-8.
Weidema, A. F., Dixon, S. J., and Sims, S. M. (2001) Activation of P2Y but not P2X(4) nucleotide receptors causes elevation of [Ca2+]i in mammalian osteoclasts Am J Physiol Cell Physiol 280, C1531-9.
Xu, S. W., Holmes, A., Leask, A., Abraham, D. J., and Black, C. M. (2001) Role of MAP kinase and PI(3) kinase signaling pathways in TNF[alpha] mediated repression of the TGF[beta] induction of CTGF Arthritis and Rheumatism 44, S192-S192.
Zhu, X. L., Ganss, B., Goldberg, H. A., and Sodek, J. (2001) Synthesis and processing of bone sialoproteins during de novo bone formation in vitro Biochem Cell Biol 79, 737-46.